› 4g63

X-ray diffraction
2.7Å resolution

Crystal structure of cytosolic IMP-GMP specific 5'-nucleotidase (lpg0095) in complex with phosphate ions from Legionella pneumophila, Northeast Structural Genomics Consortium Target LgR1

Released:
DOI: 10.2210/pdb4g63/pdb
PDB entry 4g63 coloured by chain, front view.
PDB entry 4g63 coloured by chain, side view.
PDB entry 4g63 coloured by chain, top view.
Source organism: Legionella pneumophila subsp. pneumophila str. Philadelphia 1
Primary publication:
Allosteric regulation and substrate activation in cytosolic nucleotidase II from Legionella pneumophila.
Srinivasan B, Forouhar F, Shukla A, Sampangi C, Kulkarni S, Abashidze M, Seetharaman J, Lew S, Mao L, Acton TB, Xiao R, Everett JK, Montelione GT, Tong L, Balaram H
FEBS J 281 1613-1628 (2014)
PMID: 24456211

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178740 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytosolic IMP-GMP specific 5'-nucleotidase Chain: A
Molecule details ›
Chain: A
Length: 470 amino acids
Theoretical weight: 54.74 KDa
Source organism: Legionella pneumophila subsp. pneumophila str. Philadelphia 1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5ZZB6 (Residues: 1-459; Coverage: 100%)
Gene name: lpg0095
Sequence domains: 5' nucleotidase family
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand PO4 2 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: I4122
Unit cell:
a: 154.089Å b: 154.089Å c: 191.232Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.222 0.258
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Chemical space of Escherichia coli dihydrofolate reductase inhibitors: New approaches for discovering novel drugs for old bugs.
Srinivasan et al. (2019)
2 more

1 mention without citation

Allosteric regulation and substrate activation in cytosolic nucleotidase II from Legionella pneumophila.
Srinivasan et al. (2014)