› 4g9p

X-ray diffraction
1.55Å resolution

Structure of the GcpE-MEcPP (IspG) complex from Thermus thermophilus

Released:
DOI: 10.2210/pdb4g9p/pdb
PDB entry 4g9p coloured by chain, front view.
PDB entry 4g9p coloured by chain, side view.
PDB entry 4g9p coloured by chain, top view.
Source organism: Thermus thermophilus HB27
Primary publication:
Structure of the GcpE (IspG)-MEcPP complex from Thermus thermophilus.
Rekittke I, Jomaa H, Ermler U
FEBS Lett 586 3452-7 (2012)
PMID: 22967895

Function and Biology Details

Reaction catalysed: 
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H(2)O + oxidized flavodoxin = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + reduced flavodoxin
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180818 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) Chain: A
Molecule details ›
Chain: A
Length: 406 amino acids
Theoretical weight: 44.28 KDa
Source organism: Thermus thermophilus HB27
Expression system: Escherichia coli
UniProt:
  • Canonical: Q72H18 (Residues: 1-406; Coverage: 100%)
Gene names: TT_C1677, gcpE, ispG
Sequence domains: GcpE protein
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand SF4 1 x SF4
Ligand CDI 1 x CDI
Ligand MES 2 x MES
Ligand CL 2 x CL
Ligand K 1 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P6522
Unit cell:
a: 63.65Å b: 63.65Å c: 442.27Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.178 0.177 0.202
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Mechanistic aspects of carotenoid biosynthesis.
Moise et al. (2014)
2 more

5 mentions without citation

Bioorganometallic chemistry with IspG and IspH: structure, function, and inhibition of the [Fe(4)S(4)] proteins involved in isoprenoid biosynthesis.
Wang et al. (2014)
4 more