› 4gbm

X-ray diffraction
1.62Å resolution

Sulfotransferase Domain from the Curacin Biosynthetic Pathway

Released:
DOI: 10.2210/pdb4gbm/pdb
PDB entry 4gbm coloured by chain, front view.
PDB entry 4gbm coloured by chain, side view.
PDB entry 4gbm coloured by chain, top view.
Source organism: Moorena producens
Primary publication:
Structural basis of functional group activation by sulfotransferases in complex metabolic pathways.
McCarthy JG, Eisman EB, Kulkarni S, Gerwick L, Gerwick WH, Wipf P, Sherman DH, Smith JL
ACS Chem Biol 7 1994-2003 (2012)
PMID: 22991895

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123518 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CurM Chain: A
Molecule details ›
Chain: A
Length: 323 amino acids
Theoretical weight: 36.41 KDa
Source organism: Moorena producens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: F4Y423 (Residues: 1598-1917; Coverage: 15%)
Gene name: LYNGBM3L_74580
Sequence domains: Sulfotransferase family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

5 bound ligands:
Ligand ZN 5 x ZN
Ligand A3P 1 x A3P
Ligand CL 9 x CL
Ligand SO4 1 x SO4
Ligand P6G 2 x P6G
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P212121
Unit cell:
a: 45.856Å b: 67.299Å c: 118.016Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.16 0.158 0.182
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

The Uncommon Enzymology of Cis-Acyltransferase Assembly Lines.
Keatinge-Clay AT. (2017)
6 more

2 mentions without citation

Structural basis of functional group activation by sulfotransferases in complex metabolic pathways.
McCarthy et al. (2012)
1 more