› 4gk9

X-ray diffraction
1.9Å resolution

Crystal structure of Burkholderia oklahomensis agglutinin (BOA) bound to 3a,6a-mannopentaose

Released:
DOI: 10.2210/pdb4gk9/pdb
PDB entry 4gk9 coloured by chain, front view.
PDB entry 4gk9 coloured by chain, side view.
PDB entry 4gk9 coloured by chain, top view.
Source organism: Burkholderia oklahomensis
Primary publication:
Burkholderia oklahomensis agglutinin is a canonical two-domain OAA-family lectin: structures, carbohydrate binding and anti-HIV activity.
Whitley MJ, Furey W, Kollipara S, Gronenborn AM
FEBS J 280 2056-67 (2013)
PMID: 23480609

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195737 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
OAA-family lectin sugar binding domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 279 amino acids
Theoretical weight: 29.05 KDa
Source organism: Burkholderia oklahomensis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: R4GRU1 (Residues: 1-279; Coverage: 100%)
Sequence domains: OAA-family lectin sugar binding domain
Structure domains: Lipocalin

Ligands and Environments

Carbohydrate polymer : NEW Components: BMA, MAN
Carbohydrate polymer
2 bound ligands:
Ligand NA 1 x NA
Ligand IMD 1 x IMD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E SUPERBRIGHT
Spacegroup: P43212
Unit cell:
a: 75.622Å b: 75.622Å c: 132.321Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.165 0.193
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Antiviral lectins: Selective inhibitors of viral entry.
Mitchell et al. (2017)
5 more

5 mentions without citation

Man-Specific, GalNAc/T/Tn-Specific and Neu5Ac-Specific Seaweed Lectins as Glycan Probes for the SARS-CoV-2 (COVID-19) Coronavirus.
Barre et al. (2020)
4 more