› 4gm0

X-ray diffraction
1.07Å resolution

Crystal Structure of Benzoylformate Decarboxylase Mutant L403N

Released:
DOI: 10.2210/pdb4gm0/pdb
PDB entry 4gm0 coloured by chain, front view.
PDB entry 4gm0 coloured by chain, side view.
PDB entry 4gm0 coloured by chain, top view.
Source organism: Pseudomonas putida
Primary publication:
A bulky hydrophobic residue is not required to maintain the V-conformation of enzyme-bound thiamin diphosphate.
Andrews FH, Tom AR, Gunderman PR, Novak WR, McLeish MJ
Biochemistry 52 3028-30 (2013)
PMID: 23607689

Function and Biology Details

Reaction catalysed: 
Benzoylformate = benzaldehyde + CO(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149084 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Benzoylformate decarboxylase Chain: A
Molecule details ›
Chain: A
Length: 536 amino acids
Theoretical weight: 57.48 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P20906 (Residues: 1-528; Coverage: 100%)
Gene name: mdlC
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand TZD 1 x TZD
3 bound ligands:
Ligand CA 2 x CA
Ligand GOL 2 x GOL
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: I222
Unit cell:
a: 81.445Å b: 95.685Å c: 136.881Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.138 0.137 0.151
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Acetohydroxyacid synthases: evolution, structure, and function.
Liu et al. (2016)
1 more