› 4gm6

X-ray diffraction
2Å resolution

Crystal structure of PfkB family carbohydrate kinase(TARGET EFI-502146 FROM Listeria grayi DSM 20601

Released:
DOI: 10.2210/pdb4gm6/pdb
PDB entry 4gm6 coloured by chain, front view.
PDB entry 4gm6 coloured by chain, side view.
PDB entry 4gm6 coloured by chain, top view.
Source organism: Listeria grayi DSM 20601
Entry authors: Patskovsky Y, Toro R, Bhosle R, Hillerich B, Seidel RD, Washington E, Scott Glenn A, Chowdhury S, Evans B, Hammonds J, Zencheck WD, Imker HJ, Gerlt JA, Almo SC, Enzyme Function Initiative (EFI)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112887 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carbohydrate kinase PfkB domain-containing protein Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 351 amino acids
Theoretical weight: 39.37 KDa
Source organism: Listeria grayi DSM 20601
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: D7UWR6 (Residues: 1-328; Coverage: 100%)
Gene name: HMPREF0556_10677
Sequence domains: pfkB family carbohydrate kinase
Structure domains: UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase

Ligands and Environments

2 bound ligands:
Ligand GOL 5 x GOL
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21
Unit cell:
a: 107.832Å b: 109.862Å c: 109.592Å
α: 90° β: 101.92° γ: 90°
R-values:
R R work R free
0.176 0.175 0.218
Expression system: Escherichia coli BL21(DE3)

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