› 4gs1

X-ray diffraction
1.7Å resolution

Crystal structure of DyP-type peroxidase from Thermobifida cellulosilytica

Released:
DOI: 10.2210/pdb4gs1/pdb
PDB entry 4gs1 coloured by chain, front view.
PDB entry 4gs1 coloured by chain, side view.
PDB entry 4gs1 coloured by chain, top view.
Source organism: Thermobifida cellulosilytica
Entry authors: Lukk T, Hetta AMA, Jones A, Solbiati J, Majumdar S, Cronan JE, Gerlt JA, Nair SK

Function and Biology Details

Reaction catalysed: 
Reactive Blue 5 + 2 H(2)O(2) = phthalate + 2,2'-disulfonyl azobenzene + 3-((4-amino-6-chloro-1,3,5-triazin-2-yl)amino)benzenesulfonate + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-197538 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DyP-type peroxidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 395 amino acids
Theoretical weight: 42.41 KDa
Source organism: Thermobifida cellulosilytica
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: U3KRF5 (Residues: 1-395; Coverage: 100%)
Sequence domains:

Ligands and Environments


Cofactor: Ligand HEM 2 x HEM
2 bound ligands:
Ligand OXY 2 x OXY
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P212121
Unit cell:
a: 87.68Å b: 91.19Å c: 110.55Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.146 0.174
Expression system: Escherichia coli BL21(DE3)

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