› 4gvc

X-ray diffraction
1.54Å resolution

Crystal Structure of T-cell Lymphoma Invasion and Metastasis-1 PDZ in complex with phosphorylated Syndecan1 Peptide

Released:
DOI: 10.2210/pdb4gvc/pdb
PDB entry 4gvc coloured by chain, front view.
PDB entry 4gvc coloured by chain, side view.
PDB entry 4gvc coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The structure of the Tiam1 PDZ domain/ phospho-syndecan1 complex reveals a ligand conformation that modulates protein dynamics.
Liu X, Shepherd TR, Murray AM, Xu Z, Fuentes EJ
Structure 21 342-54 (2013)
PMID: 23395182

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148451 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Rho guanine nucleotide exchange factor TIAM1 Chain: A
Molecule details ›
Chain: A
Length: 94 amino acids
Theoretical weight: 10.15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q13009 (Residues: 841-930; Coverage: 6%)
Gene name: TIAM1
Sequence domains: PDZ domain
Structure domains: Pdz3 Domain
Syndecan-1 Chain: B
Molecule details ›
Chain: B
Length: 8 amino acids
Theoretical weight: 1.1 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P18827 (Residues: 303-310; Coverage: 3%)
Gene names: SDC, SDC1

Ligands and Environments

3 bound ligands:
Ligand CL 2 x CL
Ligand ANS 1 x ANS
Ligand NA 1 x NA
1 modified residue:
Ligand PTR 1 x PTR

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: P212121
Unit cell:
a: 26.717Å b: 50.135Å c: 57.712Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.167 0.166 0.2
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

4 review citations

PDZ Domains as Drug Targets.
Christensen et al. (2019)
3 more

5 mentions without citation

Emerging Themes in PDZ Domain Signaling: Structure, Function, and Inhibition.
Liu et al. (2019)
4 more