› 4gx0

X-ray diffraction
2.6Å resolution

Crystal structure of the GsuK L97D mutant

Released:
DOI: 10.2210/pdb4gx0/pdb
PDB entry 4gx0 coloured by chain, front view.
PDB entry 4gx0 coloured by chain, side view.
PDB entry 4gx0 coloured by chain, top view.
Source organism: Geobacter sulfurreducens PCA
Primary publication:
Distinct gating mechanisms revealed by the structures of a multi-ligand gated K(+) channel.
Kong C, Zeng W, Ye S, Chen L, Sauer DB, Lam Y, Derebe MG, Jiang Y
Elife 1 e00184 (2012)
PMID: 23240087

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181064 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
TrkA domain protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 565 amino acids
Theoretical weight: 61.83 KDa
Source organism: Geobacter sulfurreducens PCA
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q74FS9 (Residues: 9-564; Coverage: 99%)
Gene name: GSU0527
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
5 bound ligands:
Ligand K 12 x K
Ligand ZN 4 x ZN
Ligand CA 4 x CA
Ligand PO4 5 x PO4
Ligand GLC 3 x GLC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: C2
Unit cell:
a: 232.934Å b: 111.67Å c: 164.133Å
α: 90° β: 134.47° γ: 90°
R-values:
R R work R free
0.205 0.203 0.249
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

BK channels: multiple sensors, one activation gate.
Yang et al. (2015)
6 more

2 mentions without citation

Distinct gating mechanisms revealed by the structures of a multi-ligand gated K(+) channel.
Kong et al. (2012)
1 more