› 4h3e

X-ray diffraction
2.25Å resolution

Crystal structure of a putative iron superoxide dismutase from Trypanosoma cruzi bound to iron

Released:
DOI: 10.2210/pdb4h3e/pdb
PDB entry 4h3e coloured by chain, front view.
PDB entry 4h3e coloured by chain, side view.
PDB entry 4h3e coloured by chain, top view.
Source organism: Trypanosoma cruzi strain CL Brener
Primary publication:
Iron superoxide dismutases in eukaryotic pathogens: new insights from Apicomplexa and Trypanosoma structures.
Phan IQ, Davies DR, Moretti NS, Shanmugam D, Cestari I, Anupama A, Fairman JW, Edwards TE, Stuart K, Schenkman S, Myler PJ
Acta Crystallogr F Struct Biol Commun 71 615-21 (2015)
PMID: 25961325

Function and Biology Details

Reaction catalysed: 
2 superoxide + 2 H(+) = O(2) + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175471 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Superoxide dismutase Chains: A, B
Molecule details ›
Chains: A, B
Length: 241 amino acids
Theoretical weight: 27.24 KDa
Source organism: Trypanosoma cruzi strain CL Brener
Expression system: Escherichia coli
UniProt:
  • Canonical: Q4DCQ3 (Residues: 1-233; Coverage: 100%)
Gene name: Tc00.1047053509775.40
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand FE2 2 x FE2
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: P212121
Unit cell:
a: 47.65Å b: 75.68Å c: 117.45Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.171 0.212
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Redox Balance Keepers and Possible Cell Functions Managed by Redox Homeostasis in Trypanosoma cruzi.
Mesías et al. (2019)
3 more

5 mentions without citation

Parallel Molecular Evolution of Catalases and Superoxide Dismutases-Focus on Thermophilic Fungal Genomes.
Chovanová et al. (2020)
4 more