› 4h7p

X-ray diffraction
1.3Å resolution

Crystal structure of a putative Cytosolic malate dehydrogenase from Leishmania major Friedlin

Released:
DOI: 10.2210/pdb4h7p/pdb
PDB entry 4h7p coloured by chain, front view.
PDB entry 4h7p coloured by chain, side view.
PDB entry 4h7p coloured by chain, top view.
Source organism: Leishmania major strain Friedlin
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed: 
(S)-malate + NAD(+) = oxaloacetate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175685 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Malate dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 345 amino acids
Theoretical weight: 36.48 KDa
Source organism: Leishmania major strain Friedlin
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q4Q7X6 (Residues: 1-324; Coverage: 100%)
Gene names: LMJF_28_2860, cMDH
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 5 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P21
Unit cell:
a: 64.58Å b: 65.66Å c: 77.65Å
α: 90° β: 110.11° γ: 90°
R-values:
R R work R free
0.153 0.152 0.178
Expression system: Escherichia coli BL21(DE3)

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