› 4hbn

X-ray diffraction
2.6Å resolution

Crystal structure of the human HCN4 channel C-terminus carrying the S672R mutation

Released:
DOI: 10.2210/pdb4hbn/pdb
PDB entry 4hbn coloured by chain, front view.
PDB entry 4hbn coloured by chain, side view.
PDB entry 4hbn coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Local and global interpretations of a disease-causing mutation near the ligand entry path in hyperpolarization-activated cAMP-gated channel.
Xu X, Marni F, Wu S, Su Z, Musayev F, Shrestha S, Xie C, Gao W, Liu Q, Zhou L
Structure 20 2116-23 (2012)
PMID: 23103389

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195215 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4 Chain: A
Molecule details ›
Chain: A
Length: 205 amino acids
Theoretical weight: 24.21 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9Y3Q4 (Residues: 521-724; Coverage: 17%)
Gene name: HCN4
Sequence domains: Cyclic nucleotide-binding domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CMP 1 x CMP
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: I422
Unit cell:
a: 95.467Å b: 95.467Å c: 115.99Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.259 0.257 0.297
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

The Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels: from Biophysics to Pharmacology of a Unique Family of Ion Channels.
Sartiani et al. (2017)
5 more

3 mentions without citation

Whole exome analysis identifies frequent CNGA1 mutations in Japanese population with autosomal recessive retinitis pigmentosa.
Katagiri et al. (2014)
2 more