› 4hcx

X-ray diffraction
2.18Å resolution

Structure of ICDH-1 from M.tuberculosis complexed with NADPH & Mn2+

Released:
DOI: 10.2210/pdb4hcx/pdb
PDB entry 4hcx coloured by chain, front view.
PDB entry 4hcx coloured by chain, side view.
PDB entry 4hcx coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Structural, kinetic and chemical mechanism of isocitrate dehydrogenase-1 from Mycobacterium tuberculosis.
Quartararo CE, Hazra S, Hadi T, Blanchard JS
Biochemistry 52 1765-75 (2013)
PMID: 23409873

Function and Biology Details

Reaction catalysed: 
(1a) isocitrate + NADP(+) = 2-oxalosuccinate + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161754 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Isocitrate dehydrogenase [NADP] Chains: A, B
Molecule details ›
Chains: A, B
Length: 409 amino acids
Theoretical weight: 45.57 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WKL1 (Residues: 1-409; Coverage: 100%)
Gene names: MTV016.39c, Rv3339c, icd
Sequence domains: Isocitrate/isopropylmalate dehydrogenase
Structure domains: Isopropylmalate Dehydrogenase

Ligands and Environments


Cofactor: Ligand NDP 2 x NDP
2 bound ligands:
Ligand MN 2 x MN
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 90.135Å b: 92.704Å c: 102.894Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.205 0.262
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Combining solvent isotope effects with substrate isotope effects in mechanistic studies of alcohol and amine oxidation by enzymes.
Fitzpatrick PF. (2015)
1 more

5 mentions without citation

Novel type II and monomeric NAD+ specific isocitrate dehydrogenases: phylogenetic affinity, enzymatic characterization, and evolutionary implication.
Wang et al. (2015)
4 more