› 4hmy

X-ray diffraction
7Å resolution

Structural basis for recruitment and activation of the AP-1 clathrin adaptor complex by Arf1

Released:
DOI: 10.2210/pdb4hmy/pdb
PDB entry 4hmy coloured by chain, front view.
PDB entry 4hmy coloured by chain, side view.
PDB entry 4hmy coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for recruitment and activation of the AP-1 clathrin adaptor complex by Arf1.
Ren X, Farías GG, Canagarajah BJ, Bonifacino JS, Hurley JH
Cell 152 755-67 (2013)
PMID: 23415225

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-149824 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
AP-1 complex subunit gamma-1 Chain: A
Molecule details ›
Chain: A
Length: 601 amino acids
Theoretical weight: 68.19 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P22892 (Residues: 1-595; Coverage: 72%)
Gene names: Adtg, Ap1g1, Clapg1
Sequence domains: Adaptin N terminal region
AP-1 complex subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 586 amino acids
Theoretical weight: 66.19 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q10567 (Residues: 1-584; Coverage: 62%)
Gene names: ADTB1, AP1B1, BAM22, CLAPB2
Sequence domains: Adaptin N terminal region
AP-1 complex subunit mu-1 Chain: M
Molecule details ›
Chain: M
Length: 423 amino acids
Theoretical weight: 48.61 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P35585 (Residues: 1-423; Coverage: 100%)
Gene names: Ap1m1, Cltnm
Sequence domains:
AP-1 complex subunit sigma-3 Chain: S
Molecule details ›
Chain: S
Length: 154 amino acids
Theoretical weight: 18.32 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q96PC3 (Residues: 1-154; Coverage: 100%)
Gene name: AP1S3
Sequence domains: Clathrin adaptor complex small chain
ADP-ribosylation factor 1 Chain: C
Molecule details ›
Chain: C
Length: 172 amino acids
Theoretical weight: 19.9 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P84077 (Residues: 17-181; Coverage: 91%)
Gene name: ARF1
Sequence domains: ADP-ribosylation factor family

Ligands and Environments

2 bound ligands:
Ligand GTP 1 x GTP
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P64
Unit cell:
a: 267.5Å b: 267.5Å c: 191.4Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.2 0.2 0.25
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

29 review citations

Forty Years of Clathrin-coated Vesicles.
Robinson MS. (2015)
28 more

18 mentions without citation

Cargo recognition in clathrin-mediated endocytosis.
Traub et al. (2013)
17 more