› 4hpl

X-ray diffraction
2Å resolution

PCGF1 Ub fold (RAWUL)/BCOR PUFD Complex

Released:
DOI: 10.2210/pdb4hpl/pdb
PDB entry 4hpl coloured by chain, front view.
PDB entry 4hpl coloured by chain, side view.
PDB entry 4hpl coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the polycomb group protein PCGF1 in complex with BCOR reveals basis for binding selectivity of PCGF homologs.
Junco SE, Wang R, Gaipa JC, Taylor AB, Schirf V, Gearhart MD, Bardwell VJ, Demeler B, Hart PJ, Kim CA
Structure 21 665-71 (2013)
PMID: 23523425

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180481 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
BCL-6 corepressor Chain: A
Molecule details ›
Chain: A
Length: 119 amino acids
Theoretical weight: 13.68 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6W2J9 (Residues: 1634-1748; Coverage: 7%)
Gene names: BCOR, KIAA1575
Sequence domains: BCORL-PCGF1-binding domain
Structure domains: BCL-6 corepressor, PCGF1 binding domain
Polycomb group RING finger protein 1 Chain: B
Molecule details ›
Chain: B
Length: 93 amino acids
Theoretical weight: 11.07 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9BSM1 (Residues: 167-255; Coverage: 34%)
Gene names: NSPC1, PCGF1, RNF68
Sequence domains: RAWUL domain RING finger- and WD40-associated ubiquitin-like
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: P62
Unit cell:
a: 67.681Å b: 67.681Å c: 75.742Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.217 0.212 0.261
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

17 review citations

BCOR involvement in cancer.
Astolfi et al. (2019)
16 more

4 mentions without citation

Molecular architecture of polycomb repressive complexes.
Chittock et al. (2017)
3 more