› 4hqc

X-ray diffraction
2.05Å resolution

Crystal structure of a green-to-red photoconvertible DRONPA, pcDRONPA in the red-on-state

Released:
DOI: 10.2210/pdb4hqc/pdb
PDB entry 4hqc coloured by chain, front view.
PDB entry 4hqc coloured by chain, side view.
PDB entry 4hqc coloured by chain, top view.
Source organism: Echinophyllia sp. SC22
Primary publication:
Green-to-red photoconvertible Dronpa mutant for multimodal super-resolution fluorescence microscopy.
Moeyaert B, Nguyen Bich N, De Zitter E, Rocha S, Clays K, Mizuno H, van Meervelt L, Hofkens J, Dedecker P
ACS Nano 8 1664-73 (2014)
PMID: 24410188

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178341 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fluorescent protein Dronpa Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 258 amino acids
Theoretical weight: 29.42 KDa
Source organism: Echinophyllia sp. SC22
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5TLG6 (Residues: 1-224; Coverage: 100%)
Gene name: Dronpa
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

3 bound ligands:
Ligand PGE 3 x PGE
Ligand PEG 2 x PEG
Ligand PG4 1 x PG4
3 modified residues:
Ligand NFA 6 x NFA
Ligand CR8 4 x CR8
Ligand IEY 6 x IEY

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Unit cell:
a: 71.212Å b: 106.131Å c: 178.929Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.178 0.233
Expression system: Escherichia coli

Quick links

Citations

12 review citations

An introduction to optical super-resolution microscopy for the adventurous biologist.
Vangindertael et al. (2018)
11 more