› 4hrh

X-ray diffraction
3Å resolution

Crystal Structure of p11-Annexin A2(N-terminal) Fusion Protein in Complex with SMARCA3 Peptide

Released:
DOI: 10.2210/pdb4hrh/pdb
PDB entry 4hrh coloured by chain, front view.
PDB entry 4hrh coloured by chain, side view.
PDB entry 4hrh coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
SMARCA3, a chromatin-remodeling factor, is required for p11-dependent antidepressant action.
Oh YS, Gao P, Lee KW, Ceglia I, Seo JS, Zhang X, Ahn JH, Chait BT, Patel DJ, Kim Y, Greengard P
Cell 152 831-43 (2013)
PMID: 23415230

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero tetramer
hetero dimer (preferred)
hetero dodecamer
Assembly name:
PDBe Complex ID:
PDB-CPX-139537 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Annexin A2; Protein S100-A10 Chains: A, B
Molecule details ›
Chains: A, B
Length: 117 amino acids
Theoretical weight: 13.44 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P60903 (Residues: 1-93; Coverage: 96%)
  • Canonical: P07355 (Residues: 2-16; Coverage: 4%)
Gene names: ANX2, ANX2L4, ANX2LG, ANXA2, CAL1H, CAL1L, CLP11, LPC2D, S100A10
Sequence domains: S-100/ICaBP type calcium binding domain
Structure domains: EF-hand
Helicase-like transcription factor Chains: C, D
Molecule details ›
Chains: C, D
Length: 14 amino acids
Theoretical weight: 1.81 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q14527 (Residues: 26-39; Coverage: 1%)
Gene names: HIP116A, HLTF, RNF80, SMARCA3, SNF2L3, ZBU1

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P4332
Unit cell:
a: 145.106Å b: 145.106Å c: 145.106Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.22 0.263
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

19 review citations

The enigmatic mossy cell of the dentate gyrus.
Scharfman HE. (2016)
18 more

1 mention without citation

SMARCA3, a chromatin-remodeling factor, is required for p11-dependent antidepressant action.
Oh et al. (2013)