› 4i1a

X-ray diffraction
2.44Å resolution

Crystal Structure of the Apo Form of RapI

Released:
DOI: 10.2210/pdb4i1a/pdb
PDB entry 4i1a coloured by chain, front view.
PDB entry 4i1a coloured by chain, side view.
PDB entry 4i1a coloured by chain, top view.
Source organism: Bacillus subtilis subsp. subtilis str. 168
Primary publication:
Conformational change-induced repeat domain expansion regulates Rap phosphatase quorum-sensing signal receptors.
Parashar V, Jeffrey PD, Neiditch MB
PLoS Biol 11 e1001512 (2013)
PMID: 23526881

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-161235 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Response regulator aspartate phosphatase I Chains: A, B
Molecule details ›
Chains: A, B
Length: 391 amino acids
Theoretical weight: 46.63 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P96649 (Residues: 1-391; Coverage: 100%)
Gene names: BSU05010, rapI, yddL
Sequence domains:
Structure domains: Tetratricopeptide repeat domain

Ligands and Environments

1 bound ligand:
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21212
Unit cell:
a: 132.897Å b: 141.209Å c: 50.532Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.221 0.27
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

Spore formation in Bacillus subtilis.
Tan et al. (2014)
8 more

6 mentions without citation

Structural Mechanisms of Peptide Recognition and Allosteric Modulation of Gene Regulation by the RRNPP Family of Quorum-Sensing Regulators.
Do et al. (2016)
5 more