› 4i3f

X-ray diffraction
1.69Å resolution

Crystal structure of serine hydrolase CCSP0084 from the polyaromatic hydrocarbon (PAH)-degrading bacterium Cycloclasticus zankles

Released:
DOI: 10.2210/pdb4i3f/pdb
PDB entry 4i3f coloured by chain, front view.
PDB entry 4i3f coloured by chain, side view.
PDB entry 4i3f coloured by chain, top view.
Source organism: Cycloclasticus sp. P1
Primary publication:
Single residues dictate the co-evolution of dual esterases: MCP hydrolases from the α/β hydrolase family.
Alcaide M, Tornés J, Stogios PJ, Xu X, Gertler C, Di Leo R, Bargiela R, Lafraya A, Guazzaroni ME, López-Cortés N, Chernikova TN, Golyshina OV, Nechitaylo TY, Plumeier I, Pieper DH, Yakimov MM, Savchenko A, Golyshin PN, Ferrer M
Biochem J 454 157-66 (2013)
PMID: 23750508

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125471 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AB hydrolase-1 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 303 amino acids
Theoretical weight: 34.01 KDa
Source organism: Cycloclasticus sp. P1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: K0C6T6 (Residues: 1-282; Coverage: 100%)
Gene name: Q91_2195
Sequence domains: alpha/beta hydrolase fold
Structure domains: alpha/beta hydrolase

Ligands and Environments

3 bound ligands:
Ligand CL 2 x CL
Ligand NA 1 x NA
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E SUPERBRIGHT
Spacegroup: C2
Unit cell:
a: 81.543Å b: 49.691Å c: 67.995Å
α: 90° β: 105.89° γ: 90°
R-values:
R R work R free
0.148 0.146 0.187
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Estimating the success of enzyme bioprospecting through metagenomics: current status and future trends.
Ferrer et al. (2016)
1 more