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Crystal structure of the RRM domain of RNA helicase HERA from T. thermophilus in complex with GGGC RNA

X-ray diffraction
2.33Å resolution
PDB entry 4i67 coloured by chain, front view.
PDB entry 4i67 coloured by chain, side view.
PDB entry 4i67 coloured by chain, top view.
Source organism: Thermus thermophilus
Assembly composition:
hetero dimer (preferred)
Entry contents: 1 distinct polypeptide molecule
1 distinct RNA molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Heat resistant RNA dependent ATPase and RNA
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Multimeric state: hetero dimer
Accessible surface area: 5339.26 Å2
Buried surface area: 1094.43 Å2
Dissociation area: 547.21 Å2
Dissociation energy (ΔGdiss): -1.57 kcal/mol
Dissociation entropy (TΔSdiss): 7.74 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-180805

Macromolecules

Heat resistant RNA dependent ATPase
Chain: A
Length: 87 amino acids
Theoretical weight: 9.58 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q72GF3 (Residues: 431-517; Coverage: 17%)
Gene name: TT_C1895
CATH: Alpha-Beta Plaits
Heat resistant RNA dependent ATPase in PDB entry 4i67, assembly 1, front view.
Heat resistant RNA dependent ATPase in PDB entry 4i67, assembly 1, side view.
Heat resistant RNA dependent ATPase in PDB entry 4i67, assembly 1, top view.
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RNA chain B
Name: 5'-R(P*GP*GP*GP*(RPC))-3'
Representative chains: B
Source organism: Thermus thermophilus [274]
Expression system: Not provided
Length: 4 nucleotides
Theoretical weight: 1.38 KDa
5'-R(P*GP*GP*GP*(RPC))-3' in PDB entry 4i67, assembly 1, front view.
5'-R(P*GP*GP*GP*(RPC))-3' in PDB entry 4i67, assembly 1, side view.
5'-R(P*GP*GP*GP*(RPC))-3' in PDB entry 4i67, assembly 1, top view.
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