› 4i9w

X-ray diffraction
2.75Å resolution

Human two pore domain K+ channel TRAAK (K2P4.1) - Fab complex structure

Released:
DOI: 10.2210/pdb4i9w/pdb
PDB entry 4i9w coloured by chain, front view.
PDB entry 4i9w coloured by chain, side view.
PDB entry 4i9w coloured by chain, top view.
Source organisms:
Primary publication:
Domain-swapped chain connectivity and gated membrane access in a Fab-mediated crystal of the human TRAAK K+ channel.
Brohawn SG, Campbell EB, MacKinnon R
Proc Natl Acad Sci U S A 110 2129-34 (2013)
PMID: 23341632

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-212786 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Potassium channel subfamily K member 4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 309 amino acids
Theoretical weight: 33.59 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q9NYG8 (Residues: 1-274; Coverage: 70%)
Gene names: KCNK4, TRAAK
Sequence domains: Ion channel
Structure domains: Helix Hairpins
ANTIBODY FAB FRAGMENT LIGHT CHAIN Chains: D, F
Molecule details ›
Chains: D, F
Length: 211 amino acids
Theoretical weight: 23.04 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
ANTIBODY FAB FRAGMENT HEAVY CHAIN Chains: E, G
Molecule details ›
Chains: E, G
Length: 217 amino acids
Theoretical weight: 23.47 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand K 5 x K
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P21
Unit cell:
a: 80.887Å b: 138.905Å c: 97.461Å
α: 90° β: 94.87° γ: 90°
R-values:
R R work R free
0.203 0.201 0.236
Expression systems:
  • Komagataella pastoris
  • Mus musculus

Quick links

Citations

26 review citations

Feeling the hidden mechanical forces in lipid bilayer is an original sense.
Anishkin et al. (2014)
25 more

20 mentions without citation

Structure of potassium channels.
Kuang et al. (2015)
19 more