› 4ibc

X-ray diffraction
1.74Å resolution

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170140 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polymerase cofactor VP35 Chains: A, B
Molecule details ›
Chains: A, B
Length: 129 amino acids
Theoretical weight: 14.21 KDa
Source organism: Ebola virus - Mayinga, Zaire, 1976
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q05127 (Residues: 215-340; Coverage: 37%)
Gene name: VP35
Sequence domains: Filoviridae VP35
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand 12G 2 x 12G
Ligand DMS 1 x DMS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 18-ID
Spacegroup: P212121
Unit cell:
a: 51.947Å b: 65.902Å c: 72.04Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.191 0.25
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

Perspectives towards antiviral drug discovery against Ebola virus.
Mirza et al. (2019)
8 more

4 mentions without citation

In silico derived small molecules bind the filovirus VP35 protein and inhibit its polymerase cofactor activity.
Brown et al. (2014)
3 more