› 4ife

X-ray diffraction
3.05Å resolution

Crystal Structure of the large terminase subunit gp2 of bacterial virus Sf6 complexed with ATP

Released:
DOI: 10.2210/pdb4ife/pdb
PDB entry 4ife coloured by chain, front view.
PDB entry 4ife coloured by chain, side view.
PDB entry 4ife coloured by chain, top view.
Source organism: Shigella virus Sf6
Primary publication:
Structures of the phage Sf6 large terminase provide new insights into DNA translocation and cleavage.
Zhao H, Christensen TE, Kamau YN, Tang L
Proc Natl Acad Sci U S A 110 8075-80 (2013)
PMID: 23630261

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180730 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phage terminase large subunit N-terminal domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 490 amino acids
Theoretical weight: 55.33 KDa
Source organism: Shigella virus Sf6
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q716H3 (Residues: 1-470; Coverage: 100%)
Gene name: 2
Sequence domains: Phage terminase large subunit
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ATP 1 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P212121
Unit cell:
a: 58.176Å b: 63.396Å c: 151.212Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.208 0.279
Expression system: Escherichia coli BL21(DE3)

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Citations

7 review citations

Molecular architecture of tailed double-stranded DNA phages.
Fokine et al. (2014)
6 more

2 mentions without citation

Structures of the phage Sf6 large terminase provide new insights into DNA translocation and cleavage.
Zhao et al. (2013)
1 more