› 4iis

X-ray diffraction
2.67Å resolution

Crystal structure of a glycosylated beta-1,3-glucanase (HEV B 2), An allergen from Hevea Brasiliensis (Space group P41)

Released:
DOI: 10.2210/pdb4iis/pdb
PDB entry 4iis coloured by chain, front view.
PDB entry 4iis coloured by chain, side view.
PDB entry 4iis coloured by chain, top view.
Source organism: Hevea brasiliensis
Primary publication:
Structural analysis of the endogenous glycoallergen Hev b 2 (endo-β-1,3-glucanase) from Hevea brasiliensis and its recognition by human basophils.
Rodríguez-Romero A, Hernández-Santoyo A, Fuentes-Silva D, Palomares LA, Muñoz-Cruz S, Yépez-Mulia L, Orozco-Martínez S
Acta Crystallogr D Biol Crystallogr 70 329-41 (2014)
PMID: 24531467

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112314 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
glucan endo-1,3-beta-D-glucosidase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 316 amino acids
Theoretical weight: 35.32 KDa
Source organism: Hevea brasiliensis
UniProt:
  • Canonical: D1M8S7 (Residues: 37-352; Coverage: 94%)
Sequence domains: Glycosyl hydrolases family 17
Structure domains: Glycosidases

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
3 bound ligands:
Ligand FLC 3 x FLC
Ligand CAC 1 x CAC
Ligand NA 1 x NA
1 modified residue:
Ligand PCA 4 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: P41
Unit cell:
a: 150.118Å b: 150.118Å c: 77.331Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.202 0.22

Quick links

Citations

1 review citation

The concept of allergen-associated molecular patterns (AAMP).
Pali-Schöll et al. (2016)
4 other articles

2 mentions without citation

Interfaces between allergen structure and diagnosis: know your epitopes.
Pomés et al. (2015)
1 more