› 4iiv

X-ray diffraction
2.5Å resolution

Crystal structure of a putative 3-oxoacyl-[acyl-carrier protein]reductase from Escherichia coli strain CFT073 complexed with NADP+ at 2.5 A resolution

Released:
DOI: 10.2210/pdb4iiv/pdb
PDB entry 4iiv coloured by chain, front view.
PDB entry 4iiv coloured by chain, side view.
PDB entry 4iiv coloured by chain, top view.
Source organism: Escherichia coli CFT073
Entry authors: Hou J, Osinski T, Zheng H, Shumilin I, Shabalin I, Shatsman S, Anderson WF, Minor W, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP(+) = 3-oxoacyl-[acyl-carrier-protein] + NADPH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101542 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-oxoacyl-[acyl-carrier protein] reductase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 267 amino acids
Theoretical weight: 28.46 KDa
Source organism: Escherichia coli CFT073
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H2V833 (Residues: 1-243; Coverage: 100%)
Gene name: c1187
Sequence domains: Enoyl-(Acyl carrier protein) reductase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments


Cofactor: Ligand NAP 4 x NAP
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P65
Unit cell:
a: 127.477Å b: 127.477Å c: 122.951Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.182 0.18 0.22
Expression system: Escherichia coli BL21(DE3)

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