› 4ij3

X-ray diffraction
2.7Å resolution

Oxidoreductase Fragment of Human QSOX1 in Complex with a FAB Fragment from an Anti- Human QSOX1 Antibody

Released:
DOI: 10.2210/pdb4ij3/pdb
PDB entry 4ij3 coloured by chain, front view.
PDB entry 4ij3 coloured by chain, side view.
PDB entry 4ij3 coloured by chain, top view.
Source organisms:
Primary publication:
An inhibitory antibody blocks the first step in the dithiol/disulfide relay mechanism of the enzyme QSOX1.
Grossman I, Alon A, Ilani T, Fass D
J Mol Biol 425 4366-78 (2013)
PMID: 23867277

Function and Biology Details

Reaction catalysed: 
2 R'C(R)SH + O(2) = R'C(R)S-S(R)CR' + H(2)O(2)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-234975 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Sulfhydryl oxidase 1 Chain: A
Molecule details ›
Chain: A
Length: 244 amino acids
Theoretical weight: 26.99 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O00391 (Residues: 33-272; Coverage: 33%)
Gene names: QSCN6, QSOX1, UNQ2520/PRO6013
Sequence domains:
Structure domains: Glutaredoxin
Light chain of FAB fragment Chain: B
Molecule details ›
Chain: B
Length: 214 amino acids
Theoretical weight: 23.64 KDa
Source organism: Mus musculus
Expression system: Not provided
Structure domains: Immunoglobulins
Heavy chain of FAB fragment Chain: C
Molecule details ›
Chain: C
Length: 221 amino acids
Theoretical weight: 23.49 KDa
Source organism: Mus musculus
Expression system: Not provided
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand PO4 2 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P61
Unit cell:
a: 209.311Å b: 209.311Å c: 55.265Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.202 0.202 0.237
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

14 citation in other articles

Ebselen inhibits QSOX1 enzymatic activity and suppresses invasion of pancreatic and renal cancer cell lines.
Hanavan et al. (2015)
13 more

2 mentions without citation

Optimizing antibody affinity and stability by the automated design of the variable light-heavy chain interfaces.
Warszawski et al. (2019)
1 more