› 4il1

X-ray diffraction
3Å resolution

Crystal Structure of the Rat Calcineurin

Released:
DOI: 10.2210/pdb4il1/pdb
PDB entry 4il1 coloured by chain, front view.
PDB entry 4il1 coloured by chain, side view.
PDB entry 4il1 coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Structural basis of calcineurin activation by calmodulin.
Ye Q, Feng Y, Yin Y, Faucher F, Currie MA, Rahman MN, Jin J, Li S, Wei Q, Jia Z
Cell Signal 25 2661-7 (2013)
PMID: 24018048

Function and Biology Details

Reaction catalysed: 
[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-144021 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Calcineurin subunit B type 1; Calmodulin-1; Protein phosphatase 3 catalytic subunit alpha Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 823 amino acids
Theoretical weight: 92.7 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0DP29 (Residues: 1-149; Coverage: 100%)
  • Canonical: P63100 (Residues: 2-170; Coverage: 99%)
  • Canonical: P63329 (Residues: 1-492; Coverage: 93%)
Gene names: CaMI, Calm, Calm1, Calna, Cam, Cam1, Cna2, Cnb, Ppp3ca, Ppp3r1
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CA 16 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P21
Unit cell:
a: 99.3Å b: 193.85Å c: 107.82Å
α: 90° β: 90.09° γ: 90°
R-values:
R R work R free
0.242 0.241 0.273
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Calcineurin.
Creamer TP. (2020)
5 more

2 mentions without citation

Cooperative autoinhibition and multi-level activation mechanisms of calcineurin.
Li et al. (2016)
1 more