› 4iqj

X-ray diffraction
3.2Å resolution

Structure of PolIIIalpha-Tauc-DNA complex suggests an atomic model of the replisome

Released:
DOI: 10.2210/pdb4iqj/pdb
PDB entry 4iqj coloured by chain, front view.
PDB entry 4iqj coloured by chain, side view.
PDB entry 4iqj coloured by chain, top view.
Source organism: Thermus aquaticus
Primary publication:
Structure of the PolIIIα-τc-DNA complex suggests an atomic model of the replisome.
Liu B, Lin J, Steitz TA
Structure 21 658-64 (2013)
PMID: 23478062

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexadecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102529 (preferred)
Entry contents:
2 distinct polypeptide molecules
3 distinct DNA molecules
Macromolecules (5 distinct):
DNA polymerase III subunit alpha Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 1220 amino acids
Theoretical weight: 137.58 KDa
Source organism: Thermus aquaticus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9XDH5 (Residues: 1-1220; Coverage: 100%)
Gene name: dnaE
Sequence domains:
Structure domains:
DNA polymerase III subunit gamma/tau Chains: M, N, O, P
Molecule details ›
Chains: M, N, O, P
Length: 177 amino acids
Theoretical weight: 19.6 KDa
Source organism: Thermus aquaticus
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0M9ACL9 (Residues: 373-540; Coverage: 31%)
Gene names: BVI061214_00485, dnaX, dnaX_2
Sequence domains: DNA polymerase III subunit tau, C-terminal domain
Structure domains: Ribonucleotide Reductase Protein R1; domain 1
DNA (5'-D(P*CP*GP*AP*AP*AP*CP*GP*AP*CP*GP*GP*CP*CP*AP*GP*TP*GP*CP*CP*A)-3') Chains: E, G, K
Molecule details ›
Chains: E, G, K
Length: 20 nucleotides
Theoretical weight: 6.14 KDa
DNA (5'-D(*TP*TP*TP*TP*TP*TP*TP*GP*TP*GP*GP*CP*AP*CP*TP*GP*GP*CP*CP*GP*TP*CP*GP*TP*TP*TP*CP*G)-3') Chains: F, H, J, L
Molecule details ›
Chains: F, H, J, L
Length: 28 nucleotides
Theoretical weight: 8.59 KDa
DNA (5'-D(P*CP*GP*AP*AP*AP*CP*GP*AP*CP*GP*GP*CP*CP*AP*GP*TP*GP*CP*CP*AP*(DOC))-3') Chain: I
Molecule details ›
Chain: I
Length: 21 nucleotides
Theoretical weight: 6.41 KDa

Ligands and Environments

2 bound ligands:
Ligand ZN 12 x ZN
Ligand MG 4 x MG
1 modified residue:
Ligand DOC 1 x DOC

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P21
Unit cell:
a: 188.53Å b: 94.97Å c: 204.08Å
α: 90° β: 89.97° γ: 90°
R-values:
R R work R free
0.266 0.264 0.305
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Interactions of small molecules with DNA junctions.
McQuaid et al. (2022)
6 other articles

2 mentions without citation

cryo-EM structures of the E. coli replicative DNA polymerase reveal its dynamic interactions with the DNA sliding clamp, exonuclease and τ.
Fernandez-Leiro et al. (2015)
1 more