› 4is4

X-ray diffraction
2.35Å resolution

The glutamine synthetase from the dicotyledonous plant M. truncatula is a decamer

Released:
DOI: 10.2210/pdb4is4/pdb
PDB entry 4is4 coloured by chain, front view.
PDB entry 4is4 coloured by chain, side view.
PDB entry 4is4 coloured by chain, top view.
Source organism: Medicago truncatula
Primary publication:
The structures of cytosolic and plastid-located glutamine synthetases from Medicago truncatula reveal a common and dynamic architecture.
Torreira E, Seabra AR, Marriott H, Zhou M, Llorca Ó, Robinson CV, Carvalho HG, Fernández-Tornero C, Pereira PJ
Acta Crystallogr D Biol Crystallogr 70 981-93 (2014)
PMID: 24699643

Function and Biology Details

Reaction catalysed: 
ATP + L-glutamate + NH(3) = ADP + phosphate + L-glutamine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo decamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126457 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamine synthetase Chains: A, B, C, D, E, F, G, H, I, J
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J
Length: 378 amino acids
Theoretical weight: 41.55 KDa
Source organism: Medicago truncatula
Expression system: Escherichia coli
UniProt:
  • Canonical: O04998 (Residues: 1-356; Coverage: 100%)
Gene names: 11427840, MTR_6g071070, MtrunA17_Chr6g0479141
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P21
Unit cell:
a: 99.335Å b: 101.672Å c: 188.084Å
α: 90° β: 103.69° γ: 90°
R-values:
R R work R free
0.177 0.175 0.217
Expression system: Escherichia coli

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Citations

2 review citations

Target-Site Mutations Conferring Herbicide Resistance.
Murphy et al. (2019)
1 more

2 mentions without citation

Isotope-Labeled RNA Building Blocks for NMR Structure and Dynamics Studies.
Olenginski et al. (2021)
1 more