› 4iso

X-ray diffraction
2.01Å resolution

Crystal Structure of Matriptase in complex with its inhibitor HAI-1

Released:
DOI: 10.2210/pdb4iso/pdb
PDB entry 4iso coloured by chain, front view.
PDB entry 4iso coloured by chain, side view.
PDB entry 4iso coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structures of matriptase in complex with its inhibitor hepatocyte growth factor activator inhibitor-1.
Zhao B, Yuan C, Li R, Qu D, Huang M, Ngo JC
J Biol Chem 288 11155-64 (2013)
PMID: 23443661

Function and Biology Details

Reaction catalysed: 
Cleaves various synthetic substrates with Arg or Lys at the P1 position and prefers small side-chain amino acids, such as Ala and Gly, at the P2 position
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128881 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Suppressor of tumorigenicity 14 protein Chain: A
Molecule details ›
Chain: A
Length: 241 amino acids
Theoretical weight: 26.48 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q9Y5Y6 (Residues: 615-855; Coverage: 28%)
Gene names: PRSS14, SNC19, ST14, TADG15
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
Kunitz-type protease inhibitor 1 Chain: B
Molecule details ›
Chain: B
Length: 60 amino acids
Theoretical weight: 6.94 KDa
Source organism: Homo sapiens
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: O43278 (Residues: 245-304; Coverage: 12%)
Gene names: HAI1, SPINT1, UNQ223/PRO256
Sequence domains: Kunitz/Bovine pancreatic trypsin inhibitor domain
Structure domains: Pancreatic trypsin inhibitor Kunitz domain

Ligands and Environments

4 bound ligands:
Ligand GSH 1 x GSH
Ligand GOL 2 x GOL
Ligand PEG 4 x PEG
Ligand PGE 1 x PGE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P43212
Unit cell:
a: 61.372Å b: 61.372Å c: 178.355Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.177 0.219
Expression systems:
  • Komagataella pastoris
  • Drosophila melanogaster

Quick links

Citations

3 review citations

Priming of SARS-CoV-2 S protein by several membrane-bound serine proteinases could explain enhanced viral infectivity and systemic COVID-19 infection.
Fuentes-Prior P. (2021)
2 more

3 mentions without citation

Functional analysis of a missense mutation in the serine protease inhibitor SPINT2 associated with congenital sodium diarrhea.
Faller et al. (2014)
2 more