› 4its

X-ray diffraction
1.85Å resolution

Crystal structure of the catalytic domain of human Pus1 with MES in the active site

Released:
DOI: 10.2210/pdb4its/pdb
PDB entry 4its coloured by chain, front view.
PDB entry 4its coloured by chain, side view.
PDB entry 4its coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
In human pseudouridine synthase 1 (hPus1), a C-terminal helical insert blocks tRNA from binding in the same orientation as in the Pus1 bacterial homologue TruA, consistent with their different target selectivities.
Czudnochowski N, Wang AL, Finer-Moore J, Stroud RM
J Mol Biol 425 3875-87 (2013)
PMID: 23707380

Function and Biology Details

Reaction catalysed: 
tRNA uridine(38-40) = tRNA pseudouridine(38-40)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-195323 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pseudouridylate synthase 1 homolog Chain: A
Molecule details ›
Chain: A
Length: 336 amino acids
Theoretical weight: 37.95 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9Y606 (Residues: 79-408; Coverage: 77%)
Gene names: PP8985, PUS1
Sequence domains: tRNA pseudouridine synthase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MES 1 x MES
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P6522
Unit cell:
a: 51.86Å b: 51.86Å c: 446.2Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.184 0.182 0.226
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Pseudouridine: still mysterious, but never a fake (uridine)!
Spenkuch et al. (2014)
5 more

3 mentions without citation

Pseudouridines in RNAs: switching atoms means shifting paradigms.
Lin et al. (2021)
2 more