› 4izn

X-ray diffraction
2.15Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178341 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fluorescent protein Dronpa Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 255 amino acids
Theoretical weight: 29.18 KDa
Source organism: Echinophyllia sp. SC22
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5TLG6 (Residues: 1-224; Coverage: 100%)
Gene name: Dronpa
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

1 bound ligand:
Ligand K 12 x K
1 modified residue:
Ligand CR8 12 x CR8

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P21
Unit cell:
a: 74.7Å b: 180.93Å c: 113.161Å
α: 90° β: 93.37° γ: 90°
R-values:
R R work R free
0.195 0.192 0.245
Expression system: Escherichia coli

Quick links

Citations

12 review citations

An introduction to optical super-resolution microscopy for the adventurous biologist.
Vangindertael et al. (2018)
11 more