› 4j00

X-ray diffraction
3Å resolution

Crystal Structure of Fischerella Transcription Factor HetR complexed with 24mer DNA target

Released:
DOI: 10.2210/pdb4j00/pdb
PDB entry 4j00 coloured by chain, front view.
PDB entry 4j00 coloured by chain, side view.
PDB entry 4j00 coloured by chain, top view.
Source organisms:
Primary publication:
Structures of complexes comprised of Fischerella transcription factor HetR with Anabaena DNA targets.
Kim Y, Ye Z, Joachimiak G, Videau P, Young J, Hurd K, Callahan SM, Gornicki P, Zhao J, Haselkorn R, Joachimiak A
Proc Natl Acad Sci U S A 110 E1716-23 (2013)
PMID: 23610410

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117669 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
HetR C-terminal Hood domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 302 amino acids
Theoretical weight: 35.23 KDa
Source organism: Fischerella thermalis PCC 7521
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q2ACK9 (Residues: 1-247; Coverage: 100%)
Gene name: hetR
Sequence domains:
Structure domains:
DNA (5'-D(*TP*GP*GP*TP*GP*AP*GP*GP*GP*GP*TP*TP*AP*AP*AP*CP*CP*CP*CP*TP*CP*AP*CP*C)-3') Chains: C, D
Molecule details ›
Chains: C, D
Length: 24 nucleotides
Theoretical weight: 7.37 KDa
Source organism: Anabaena
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P31
Unit cell:
a: 92.93Å b: 92.93Å c: 97.652Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.177 0.168 0.205
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

2 review citations

Genetic responses to carbon and nitrogen availability in Anabaena.
Herrero et al. (2019)
1 more

1 mention without citation

Structures of complexes comprised of Fischerella transcription factor HetR with Anabaena DNA targets.
Kim et al. (2013)