› 4j2n

X-ray diffraction
2.35Å resolution

Crystal Structure of mycobacteriophage Pukovnik Xis

Released:
DOI: 10.2210/pdb4j2n/pdb
PDB entry 4j2n coloured by chain, front view.
PDB entry 4j2n coloured by chain, side view.
PDB entry 4j2n coloured by chain, top view.
Source organism: Mycobacterium virus Pukovnik
Primary publication:
The structure of Xis reveals the basis for filament formation and insight into DNA bending within a mycobacteriophage intasome.
Singh S, Plaks JG, Homa NJ, Amrich CG, Héroux A, Hatfull GF, VanDemark AP
J Mol Biol 426 412-22 (2014)
PMID: 24112940

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109944 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Helix-turn-helix domain-containing protein Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 58 amino acids
Theoretical weight: 6.41 KDa
Source organism: Mycobacterium virus Pukovnik
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B3VGI6 (Residues: 1-56; Coverage: 100%)
Gene names: 37, Pukovnik_37
Sequence domains: Helix-turn-helix domain

Ligands and Environments

1 bound ligand:
Ligand SO4 8 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E DW, NSLS BEAMLINE X25
Spacegroup: C2221
Unit cell:
a: 89.423Å b: 129.985Å c: 92.346Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.264 0.226 0.267
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

When is a transcription factor a NAP?
Dorman et al. (2020)
10 other articles

2 mentions without citation

The structure of Xis reveals the basis for filament formation and insight into DNA bending within a mycobacteriophage intasome.
Singh et al. (2014)
1 more