› 4j2p

X-ray diffraction
1.85Å resolution

Crystal structure of LuxF from Photobacterium leiognathi

Released:
DOI: 10.2210/pdb4j2p/pdb
PDB entry 4j2p coloured by chain, front view.
PDB entry 4j2p coloured by chain, side view.
PDB entry 4j2p coloured by chain, top view.
Source organism: Photobacterium leiognathi
Primary publication:
Structural and biochemical properties of LuxF from Photobacterium leiognathi.
Bergner T, Tabib CR, Winkler A, Stipsits S, Kayer H, Lee J, Malthouse JP, Mayhew S, Müller F, Gruber K, Macheroux P
Biochim Biophys Acta 1854 1466-75 (2015)
PMID: 26209460

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140561 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Non-fluorescent flavoprotein Chain: A
Molecule details ›
Chain: A
Length: 236 amino acids
Theoretical weight: 27.34 KDa
Source organism: Photobacterium leiognathi
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P09142 (Residues: 1-228; Coverage: 100%)
Gene name: luxF
Sequence domains: Luciferase-like monooxygenase
Structure domains: Luciferase-like domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: I422
Unit cell:
a: 93.18Å b: 93.18Å c: 115.43Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.171 0.204
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Molecular Mechanisms of Bacterial Bioluminescence.
Brodl et al. (2018)
1 more