› 4j37

X-ray diffraction
1.75Å resolution

Function and Biology Details

Reaction catalysed: 
tRNA uridine(38-40) = tRNA pseudouridine(38-40)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195323 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pseudouridylate synthase 1 homolog Chain: A
Molecule details ›
Chain: A
Length: 336 amino acids
Theoretical weight: 37.95 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y606 (Residues: 79-408; Coverage: 77%)
Gene names: PP8985, PUS1
Sequence domains: tRNA pseudouridine synthase
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand SO4 2 x SO4
Ligand CL 1 x CL
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P6522
Unit cell:
a: 51.95Å b: 51.95Å c: 445.49Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.202 0.2 0.239
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Pseudouridine: still mysterious, but never a fake (uridine)!
Spenkuch et al. (2014)
5 more

4 mentions without citation

Regulation and Function of RNA Pseudouridylation in Human Cells.
Borchardt et al. (2020)
3 more