› 4jcx

X-ray diffraction
2.3Å resolution

Crystal structure of the Restriction-Modification Controller Protein C.Csp231I OL operator complex

Released:
DOI: 10.2210/pdb4jcx/pdb
PDB entry 4jcx coloured by chain, front view.
PDB entry 4jcx coloured by chain, side view.
PDB entry 4jcx coloured by chain, top view.
Source organism: Citrobacter sp. RFL231
Primary publication:
Structural analysis of DNA binding by C.Csp231I, a member of a novel class of R-M controller proteins regulating gene expression.
Shevtsov MB, Streeter SD, Thresh SJ, Swiderska A, McGeehan JE, Kneale GG
Acta Crystallogr D Biol Crystallogr 71 398-407 (2015)
PMID: 25664751

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117697 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
HTH cro/C1-type domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 98 amino acids
Theoretical weight: 11.38 KDa
Source organism: Citrobacter sp. RFL231
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q32WH4 (Residues: 1-98; Coverage: 100%)
Sequence domains: Helix-turn-helix domain
Structure domains: lambda repressor-like DNA-binding domains
DNA (5'-D(*AP*CP*AP*CP*TP*AP*AP*GP*GP*AP*AP*AP*AP*CP*TP*TP*AP*GP*TP*AP*A)-3') Chain: C
Molecule details ›
Chain: C
Length: 21 nucleotides
Theoretical weight: 6.47 KDa
Source organism: Citrobacter sp. RFL231
Expression system: Not provided
DNA (5'-D(*TP*TP*AP*CP*TP*AP*AP*GP*TP*TP*TP*TP*CP*CP*TP*TP*AP*GP*TP*GP*T)-3') Chain: D
Molecule details ›
Chain: D
Length: 21 nucleotides
Theoretical weight: 6.41 KDa
Source organism: Citrobacter sp. RFL231
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P61
Unit cell:
a: 62.2Å b: 62.2Å c: 147.8Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.173 0.172 0.196
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

8 citation in other articles

Insights into the inhibition of type I-F CRISPR-Cas system by a multifunctional anti-CRISPR protein AcrIF24.
Yang et al. (2022)
7 more

1 mention without citation

Structural analysis of DNA binding by C.Csp231I, a member of a novel class of R-M controller proteins regulating gene expression.
Shevtsov et al. (2015)