› 4jcy

X-ray diffraction
1.8Å resolution

Crystal structure of the Restriction-Modification Controller Protein C.Csp231I OR operator complex

Released:
DOI: 10.2210/pdb4jcy/pdb
PDB entry 4jcy coloured by chain, front view.
PDB entry 4jcy coloured by chain, side view.
PDB entry 4jcy coloured by chain, top view.
Source organism: Citrobacter sp. RFL231
Primary publication:
Structural analysis of DNA binding by C.Csp231I, a member of a novel class of R-M controller proteins regulating gene expression.
Shevtsov MB, Streeter SD, Thresh SJ, Swiderska A, McGeehan JE, Kneale GG
Acta Crystallogr D Biol Crystallogr 71 398-407 (2015)
PMID: 25664751

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117698 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
HTH cro/C1-type domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 98 amino acids
Theoretical weight: 11.38 KDa
Source organism: Citrobacter sp. RFL231
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q32WH4 (Residues: 1-98; Coverage: 100%)
Sequence domains: Helix-turn-helix domain
Structure domains: lambda repressor-like DNA-binding domains
DNA (5'-D(*AP*AP*AP*CP*TP*AP*AP*GP*AP*AP*AP*AP*TP*CP*TP*TP*AP*GP*CP*AP*A)-3') Chain: C
Molecule details ›
Chain: C
Length: 21 nucleotides
Theoretical weight: 6.46 KDa
Source organism: Citrobacter sp. RFL231
Expression system: Not provided
DNA (5'-D(*TP*TP*GP*CP*TP*AP*AP*GP*AP*TP*TP*TP*TP*CP*TP*TP*AP*GP*TP*TP*T)-3') Chain: D
Molecule details ›
Chain: D
Length: 21 nucleotides
Theoretical weight: 6.42 KDa
Source organism: Citrobacter sp. RFL231
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand IOD 4 x IOD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P61
Unit cell:
a: 62.34Å b: 62.34Å c: 158.08Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.148 0.147 0.163
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

7 citation in other articles

Insights into the inhibition of type I-F CRISPR-Cas system by a multifunctional anti-CRISPR protein AcrIF24.
Yang et al. (2022)
6 more

2 mentions without citation

Structural analysis of DNA binding by C.Csp231I, a member of a novel class of R-M controller proteins regulating gene expression.
Shevtsov et al. (2015)
1 more