› 4jgt

X-ray diffraction
2.16Å resolution

Structure and kinetic analysis of H2S production by human Mercaptopyruvate Sulfurtransferase

Released:
DOI: 10.2210/pdb4jgt/pdb
PDB entry 4jgt coloured by chain, front view.
PDB entry 4jgt coloured by chain, side view.
PDB entry 4jgt coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure and kinetic analysis of H2S production by human mercaptopyruvate sulfurtransferase.
Yadav PK, Yamada K, Chiku T, Koutmos M, Banerjee R
J Biol Chem 288 20002-13 (2013)
PMID: 23698001

Function and Biology Details

Reaction catalysed: 
(1a) 2-oxo-3-sulfanylpropanoate + [3-mercaptopyruvate sulfurtransferase]-L-cysteine = pyruvate + [3-mercaptopyruvate sulfurtransferase]-S-sulfanyl-L-cysteine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150427 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-mercaptopyruvate sulfurtransferase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 299 amino acids
Theoretical weight: 33.5 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P25325 (Residues: 11-289; Coverage: 94%)
Gene names: MPST, TST2
Sequence domains: Rhodanese-like domain
Structure domains: Rhodanese-like domain

Ligands and Environments

3 bound ligands:
Ligand PYR 3 x PYR
Ligand SO4 8 x SO4
Ligand GOL 1 x GOL
1 modified residue:
Ligand CSS 3 x CSS

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: C2
Unit cell:
a: 110.737Å b: 171.859Å c: 72.984Å
α: 90° β: 117.18° γ: 90°
R-values:
R R work R free
0.187 0.186 0.22
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

56 review citations

Regulation of mitochondrial bioenergetic function by hydrogen sulfide. Part I. Biochemical and physiological mechanisms.
Szabo et al. (2014)
55 more

15 mentions without citation

Chemical Biology of H2S Signaling through Persulfidation.
Filipovic et al. (2018)
14 more