› 4jie

X-ray diffraction
2.45Å resolution

Structural analysis and insights into glycon specificity of the rice GH1 Os7BGlu26 beta-D-mannosidase

Released:
DOI: 10.2210/pdb4jie/pdb
PDB entry 4jie coloured by chain, front view.
PDB entry 4jie coloured by chain, side view.
PDB entry 4jie coloured by chain, top view.
Source organism: Oryza sativa Indica Group
Primary publication:
Structural analysis and insights into the glycon specificity of the rice GH1 Os7BGlu26 β-D-mannosidase.
Tankrathok A, Iglesias-Fernández J, Luang S, Robinson RC, Kimura A, Rovira C, Hrmova M, Ketudat Cairns JR
Acta Crystallogr D Biol Crystallogr 69 2124-35 (2013)
PMID: 24100330

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal, non-reducing beta-D-mannose residues in beta-D-mannosides
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106989 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-glucosidase Chain: A
Molecule details ›
Chain: A
Length: 503 amino acids
Theoretical weight: 57.78 KDa
Source organism: Oryza sativa Indica Group
Expression system: Escherichia coli
UniProt:
  • Canonical: A2YPH1 (Residues: 28-510; Coverage: 100%)
Gene name: OsI_27163
Sequence domains: Glycosyl hydrolase family 1
Structure domains: Glycosidases

Ligands and Environments

3 bound ligands:
Ligand GOL 6 x GOL
Ligand EPE 1 x EPE
Ligand BMA 1 x BMA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P212121
Unit cell:
a: 68.01Å b: 73.624Å c: 133.984Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.155 0.153 0.195
Expression system: Escherichia coli

Quick links

Citations

3 citation in other articles

Structure-guided engineering of the substrate specificity of a fungal β-glucuronidase toward triterpenoid saponins.
Lv et al. (2018)
2 more