› 4jin

X-ray diffraction
2.1Å resolution

X-ray crystal structure of Archaeoglobus fulgidus Rio1 bound to (2E)-N-benzyl-2-cyano-3-(pyridine-4-yl)acrylamide (WP1086)

Released:
DOI: 10.2210/pdb4jin/pdb
PDB entry 4jin coloured by chain, front view.
PDB entry 4jin coloured by chain, side view.
PDB entry 4jin coloured by chain, top view.
Source organism: Archaeoglobus fulgidus DSM 4304
Primary publication:
Development of novel molecular probes of the Rio1 atypical protein kinase.
Mielecki M, Krawiec K, Kiburu I, Grzelak K, Zagórski W, Kierdaszuk B, Kowa K, Fokt I, Szymanski S, Swierk P, Szeja W, Priebe W, Lesyng B, LaRonde-LeBlanc N
Biochim Biophys Acta 1834 1292-301 (2013)
PMID: 23523885

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128028 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RIO-type serine/threonine-protein kinase Rio1 Chain: A
Molecule details ›
Chain: A
Length: 258 amino acids
Theoretical weight: 30.14 KDa
Source organism: Archaeoglobus fulgidus DSM 4304
Expression system: Escherichia coli
UniProt:
  • Canonical: O28471 (Residues: 1-258; Coverage: 100%)
Gene names: AF_1804, rio1
Sequence domains: RIO1 family

Ligands and Environments

1 bound ligand:
Ligand 1L7 1 x 1L7
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P21
Unit cell:
a: 42.553Å b: 52.608Å c: 63.654Å
α: 90° β: 109.22° γ: 90°
R-values:
R R work R free
0.182 0.179 0.241
Expression system: Escherichia coli

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Citations

3 review citations

The ancient microbial RIO kinases.
LaRonde NA. (2014)
2 more