› 4jnc

X-ray diffraction
1.96Å resolution

Function and Biology Details

Reactions catalysed: 
(9S,10S)-10-hydroxy-9-(phosphonooxy)octadecanoate + H(2)O = (9S,10S)-9,10-dihydroxyoctadecanoate + phosphate
An epoxide + H(2)O = a glycol
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152745 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bifunctional epoxide hydrolase 2 Chain: A
Molecule details ›
Chain: A
Length: 315 amino acids
Theoretical weight: 36.14 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P34913 (Residues: 238-549; Coverage: 56%)
Gene name: EPHX2
Sequence domains: alpha/beta hydrolase fold
Structure domains: alpha/beta hydrolase

Ligands and Environments

1 bound ligand:
Ligand 1LF 1 x 1LF
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E SUPERBRIGHT
Spacegroup: P21212
Unit cell:
a: 80.285Å b: 91.951Å c: 45.87Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.187 0.219
Expression system: Escherichia coli BL21

Quick links

Citations

3 review citations

Achievements, Challenges, and Opportunities in DNA-Encoded Library Research: An Academic Point of View.
Yuen et al. (2017)
2 more

10 mentions without citation

Prospective for cytochrome P450 epoxygenase cardiovascular and renal therapeutics.
Imig JD. (2018)
9 more