› 4jpz

X-ray diffraction
3.02Å resolution

Voltage-gated sodium channel 1.2 C-terminal domain in complex with FGF13U and Ca2+/calmodulin

Released:
DOI: 10.2210/pdb4jpz/pdb
PDB entry 4jpz coloured by chain, front view.
PDB entry 4jpz coloured by chain, side view.
PDB entry 4jpz coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural analyses of Ca²⁺/CaM interaction with NaV channel C-termini reveal mechanisms of calcium-dependent regulation.
Wang C, Chung BC, Yan H, Wang HG, Lee SY, Pitt GS
Nat Commun 5 4896 (2014)
PMID: 25232683

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-143956 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Fibroblast growth factor 13 Chains: A, E
Molecule details ›
Chains: A, E
Length: 192 amino acids
Theoretical weight: 21.61 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q92913 (Residues: 58-245; Coverage: 77%)
Gene names: FGF13, FHF2
Sequence domains: Fibroblast growth factor
Structure domains: Trefoil (Acidic Fibroblast Growth Factor, subunit A)
Sodium channel protein type 2 subunit alpha Chains: B, H
Molecule details ›
Chains: B, H
Length: 184 amino acids
Theoretical weight: 21.04 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99250 (Residues: 1777-1937; Coverage: 8%)
Gene names: HBA, NAC2, SCN2A, SCN2A1, SCN2A2
Structure domains:
Calmodulin-1 Chains: C, I
Molecule details ›
Chains: C, I
Length: 149 amino acids
Theoretical weight: 16.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0DP23 (Residues: 1-149; Coverage: 100%)
Gene names: CALM, CALM1, CAM, CAM1
Sequence domains: EF-hand domain pair

Ligands and Environments

1 bound ligand:
Ligand CA 8 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: C2
Unit cell:
a: 153.133Å b: 86.106Å c: 109.399Å
α: 90° β: 100.9° γ: 90°
R-values:
R R work R free
0.214 0.212 0.246
Expression system: Escherichia coli

Quick links

Citations

14 review citations

Towards a Unified Theory of Calmodulin Regulation (Calmodulation) of Voltage-Gated Calcium and Sodium Channels.
Ben-Johny et al. (2015)
13 more

18 mentions without citation

Current view on regulation of voltage-gated sodium channels by calcium and auxiliary proteins.
Pitt et al. (2016)
17 more