› 4jtd

X-ray diffraction
2.54Å resolution

Crystal structure of Kv1.2-2.1 paddle chimera channel in complex with Lys27Met mutant of Charybdotoxin

Released:
DOI: 10.2210/pdb4jtd/pdb
PDB entry 4jtd coloured by chain, front view.
PDB entry 4jtd coloured by chain, side view.
PDB entry 4jtd coloured by chain, top view.
Source organisms:
Primary publication:
Structure of a pore-blocking toxin in complex with a eukaryotic voltage-dependent K(+) channel.
Banerjee A, Lee A, Campbell E, Mackinnon R
Elife 2 e00594 (2013)
PMID: 23705070

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero octamer
hetero nonamer (preferred)
PDBe Complex ID:
PDB-CPX-146606 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Voltage-gated potassium channel subunit beta-2 Chains: A, G
Molecule details ›
Chains: A, G
Length: 333 amino acids
Theoretical weight: 37.35 KDa
Source organism: Rattus norvegicus
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P62483 (Residues: 36-367; Coverage: 91%)
Gene names: Ckbeta2, Kcnab2, Kcnb3
Sequence domains: Aldo/keto reductase family
Structure domains: NADP-dependent oxidoreductase domain
Potassium voltage-gated channel subfamily A member 2; Potassium voltage-gated channel subfamily B member 1 Chains: B, H
Molecule details ›
Chains: B, H
Length: 514 amino acids
Theoretical weight: 58.82 KDa
Source organism: Rattus norvegicus
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P63142 (Residues: 1-31, 33-266, 305-499; Coverage: 92%)
  • Canonical: P15387 (Residues: 30-30, 274-307; Coverage: 4%)
Gene names: Kcna2, Kcnb1
Sequence domains:
Structure domains:
Potassium channel toxin alpha-KTx 1.1 Chain: Y
Molecule details ›
Chain: Y
Length: 37 amino acids
Theoretical weight: 4.31 KDa
Source organism: Leiurus quinquestriatus hebraeus
Expression system: Escherichia coli
UniProt:
  • Canonical: P13487 (Residues: 23-59; Coverage: 100%)
Sequence domains: Scorpion short toxin, BmKK2

Ligands and Environments

3 bound ligands:
Ligand NAP 2 x NAP
Ligand K 8 x K
Ligand PGW 17 x PGW
1 modified residue:
Ligand PCA 1 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P4212
Unit cell:
a: 144.867Å b: 144.867Å c: 284.292Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.21 0.235
Expression systems:
  • Komagataella pastoris
  • Escherichia coli

Quick links

Citations

27 review citations

From foe to friend: using animal toxins to investigate ion channel function.
Kalia et al. (2015)
26 more

2 mentions without citation

Peptide toxins and small-molecule blockers of BK channels.
Yu et al. (2016)
1 more