› 4jub

X-ray diffraction
1.9Å resolution

Crystal Structure of the His70Thr mutant of Benzoylformate Decarboxylase from Pseudomonas putida

Released:
DOI: 10.2210/pdb4jub/pdb
PDB entry 4jub coloured by chain, front view.
PDB entry 4jub coloured by chain, side view.
PDB entry 4jub coloured by chain, top view.
Source organism: Pseudomonas putida
Entry authors: McLeish MJ, Brodkin HR

Function and Biology Details

Reaction catalysed: 
Benzoylformate = benzaldehyde + CO(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149084 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Benzoylformate decarboxylase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 534 amino acids
Theoretical weight: 57.19 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P20906 (Residues: 1-528; Coverage: 100%)
Gene name: mdlC
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand TPP 4 x TPP
3 bound ligands:
Ligand MG 3 x MG
Ligand CA 4 x CA
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P212121
Unit cell:
a: 70.465Å b: 161.682Å c: 175.874Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 0.166 0.203
Expression system: Escherichia coli BL21(DE3)

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