› 4jwt

X-ray diffraction
2.05Å resolution

Crystal structure of a putative 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase from Sulfurimonas denitrificans DSM 1251 (Target NYSGRC-029304 )

Released:
DOI: 10.2210/pdb4jwt/pdb
PDB entry 4jwt coloured by chain, front view.
PDB entry 4jwt coloured by chain, side view.
PDB entry 4jwt coloured by chain, top view.
Source organism: Sulfurimonas denitrificans DSM 1251
Entry authors: Sampathkumar P, Almo SC, New York Structural Genomics Research Consortium (NYSGRC)

Function and Biology Details

Reaction catalysed: 
5'-deoxyadenosine + H(2)O = 5-deoxy-D-ribose + adenine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174243 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
adenosylhomocysteine nucleosidase Chain: A
Molecule details ›
Chain: A
Length: 259 amino acids
Theoretical weight: 28.64 KDa
Source organism: Sulfurimonas denitrificans DSM 1251
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q30QR2 (Residues: 2-237; Coverage: 100%)
Gene name: Suden_1392
Sequence domains: Phosphorylase superfamily
Structure domains: Nucleoside phosphorylase domain

Ligands and Environments

2 bound ligands:
Ligand EDO 4 x EDO
Ligand ADE 1 x ADE
1 modified residue:
Ligand MSE 11 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P6522
Unit cell:
a: 77.598Å b: 77.598Å c: 228.425Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.178 0.178 0.193
Expression system: Escherichia coli BL21(DE3)

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