› 4k9o

X-ray diffraction
1.89Å resolution

Crystal Structure of the Phe397Ala mutant of Benzoylformate Decarboxylase from Pseudomonas putida

Released:
DOI: 10.2210/pdb4k9o/pdb
PDB entry 4k9o coloured by chain, front view.
PDB entry 4k9o coloured by chain, side view.
PDB entry 4k9o coloured by chain, top view.
Source organism: Pseudomonas putida
Entry authors: Brodkin HR, McLeish MJ

Function and Biology Details

Reaction catalysed: 
Benzoylformate = benzaldehyde + CO(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149084 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Benzoylformate decarboxylase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 533 amino acids
Theoretical weight: 57.02 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P20906 (Residues: 2-528; Coverage: 100%)
Gene name: mdlC
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand TPP 4 x TPP
3 bound ligands:
Ligand MG 2 x MG
Ligand CA 4 x CA
Ligand GOL 6 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P212121
Unit cell:
a: 70.183Å b: 161.218Å c: 175.204Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.167 0.167 0.192
Expression system: Escherichia coli BL21(DE3)

Quick links