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Crystal structure of probable sugar kinase protein from Rhizobium etli CFN 42 complexed with quinoline-3-carboxylic acid

X-ray diffraction
1.5Å resolution
PDB entry 4kal coloured by chain, front view.
PDB entry 4kal coloured by chain, side view.
PDB entry 4kal coloured by chain, top view.
Source organism: Rhizobium etli CFN 42
Assembly composition:
homo dimer (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Carbohydrate kinase PfkB domain-containing protein
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Multimeric state: homo dimer
Accessible surface area: 24763.47 Å2
Buried surface area: 4225.74 Å2
Dissociation area: 994.11 Å2
Dissociation energy (ΔGdiss): 3.22 kcal/mol
Dissociation entropy (TΔSdiss): 13.57 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-173687

Macromolecules

Carbohydrate kinase PfkB domain-containing protein
Chains: A, B
Length: 352 amino acids
Theoretical weight: 38.85 KDa
Source organism: Rhizobium etli CFN 42
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q2KDX6 (Residues: 1-330; Coverage: 100%)
Gene name: RHE_CH00135
Pfam: pfkB family carbohydrate kinase
InterPro:
CATH: UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase
Carbohydrate kinase PfkB domain-containing protein in PDB entry 4kal, assembly 1, front view.
Carbohydrate kinase PfkB domain-containing protein in PDB entry 4kal, assembly 1, side view.
Carbohydrate kinase PfkB domain-containing protein in PDB entry 4kal, assembly 1, top view.
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