› 4kcf

X-ray diffraction
2.1Å resolution

X-ray Structure of a KijD3 in Complex with FMN and dTDP-3-amino-2,3,6-trideoxy-4-keto-3-methyl-D-glucose

Released:
DOI: 10.2210/pdb4kcf/pdb
PDB entry 4kcf coloured by chain, front view.
PDB entry 4kcf coloured by chain, side view.
PDB entry 4kcf coloured by chain, top view.
Source organism: Actinomadura kijaniata
Primary publication:
Active site architecture of a sugar N-oxygenase.
Thoden JB, Branch MC, Zimmer AL, Bruender NA, Holden HM
Biochemistry 52 3191-3 (2013)
PMID: 23621882

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-109917 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
FAD-dependent oxidoreductase Chain: A
Molecule details ›
Chain: A
Length: 454 amino acids
Theoretical weight: 48.33 KDa
Source organism: Actinomadura kijaniata
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B3TMR1 (Residues: 1-437; Coverage: 100%)
Gene name: KijD3
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand AKM 1 x AKM
Ligand FMN 1 x FMN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: I222
Unit cell:
a: 70.828Å b: 81.297Å c: 152.628Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.175 0.225
Expression system: Escherichia coli BL21(DE3)

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Citations

3 review citations

Heteroatom-Heteroatom Bond Formation in Natural Product Biosynthesis.
Waldman et al. (2017)
2 more

2 mentions without citation

Cryptic indole hydroxylation by a non-canonical terpenoid cyclase parallels bacterial xenobiotic detoxification.
Kugel et al. (2017)
1 more