› 4kem

X-ray diffraction
1.3Å resolution

Crystal structure of a tartrate dehydratase from azospirillum, target efi-502395, with bound mg and a putative acrylate ion, ordered active site

Released:
DOI: 10.2210/pdb4kem/pdb
PDB entry 4kem coloured by chain, front view.
PDB entry 4kem coloured by chain, side view.
PDB entry 4kem coloured by chain, top view.
Source organism: Azospirillum sp. B510
Entry authors: Vetting MW, Wichelecki D, Morisco LL, Wasserman SR, Sojitra S, Stead M, Hillerich B, Love J, Seidel RD, Imker HJ, Gerlt JA, Almo SC, Enzyme Function Initiative (EFI)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112530 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mandelate racemase/muconate lactonizing enzyme C-terminal domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 390 amino acids
Theoretical weight: 42.98 KDa
Source organism: Azospirillum sp. B510
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: D3P639 (Residues: 1-390; Coverage: 100%)
Gene name: AZL_d02920
Sequence domains: Enolase C-terminal domain-like
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MG 2 x MG
Ligand CL 6 x CL
Ligand AKR 2 x AKR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P21
Unit cell:
a: 50.167Å b: 82.925Å c: 103.266Å
α: 90° β: 100.86° γ: 90°
R-values:
R R work R free
0.166 0.164 0.186
Expression system: Escherichia coli BL21(DE3)

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